Establishment of a primed pluripotent epiblast stem cell in FGF4-based conditions

Establishment of a primed pluripotent epiblast stem cell in FGF4-based conditions

Several mouse pluripotent stem cell types have been established either from mouse blastocysts and epiblasts. Among these, embryonic stem cells (ESCs) are considered to represent a "naïve", epiblast stem cells (EpiSCs) a "primed" pluripotent state. Although EpiSCs form derivatives of all three germ layers during in vitro differentiation, they rarely incorporate into the inner cell mass of blasto...

ERRATUM: Establishment of a primed pluripotent epiblast stem cell in FGF4-based conditions

Differentiation of Primed Human Pluripotent Stem Cells

Establishment of a pancreatic stem cell line from fibroblast-derived induced pluripotent stem cells

BACKGROUND For cell therapies to treat diabetes, it is important to produce a sufficient number of pancreatic endocrine cells that function similarly to primary islets. Induced pluripotent stem (iPS) cells represent a potentially unlimited source of functional pancreatic endocrine cells. However, the use of iPS cells for laboratory studies and cell-based therapies is hampered by their high tumo...

Smad2 is essential for maintenance of the human and mouse primed pluripotent stem cell state.

Human embryonic stem cells and mouse epiblast stem cells represent a primed pluripotent stem cell state that requires TGF-β/activin signaling. TGF-β and/or activin are commonly thought to regulate transcription through both Smad2 and Smad3. However, the different contributions of these two Smads to primed pluripotency and the downstream events that they may regulate remain poorly understood. We...